PPY ELISA Kit

Catalog No. ABIN5522373

Mouse PPY ELISA Kit Colorimetric assay for quantification of Mouse PPY.

Quick Overview for PPY ELISA Kit (ABIN5522373)

Target: PPY (Pancreatic Polypeptide (PPY))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details PPY ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: For quantitative detection of Ppy in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ppy. No significant cross-reactivity or interference between Ppy and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Ppy and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 18.75 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Lyophilized Standard
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody (Lyophilized)
• Purified water
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Alternative Name: Ppy

Background: PPY/INN/Obinepitide/PNP/PP/Obinepitide/pancreatic polypeptide(ppy)/pancreatic polypeptide/pancreatic polypeptide Y/pancreatic prohormone

UniProt: P10601

Pathways: Feeding Behaviour